Research Catalog

G proteins : techniques of analysis

Title
  1. G proteins : techniques of analysis / edited by David R. Manning.
Published by
  1. Boca Raton : CRC Press, 1999.

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Details

Additional authors
  1. Manning, David R.
Description
  1. 301 pages : illustrations; 25 cm.
Summary
  1. "G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility."--BOOK JACKET. "G Proteins: Techniques of Analysis includes expression and functions analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and mapping pathways established among receptors, G proteins, and effectors."--BOOK JACKET.
Series statement
  1. CRC methods in signal transduction series
Uniform title
  1. Methods in signal transduction.
Subject
  1. GTP-Binding Proteins > physiology
  2. GTP-Binding Proteins > analysis
  3. G proteins > Analysis
Contents
  1. Ch. 1. Expression and Purification of G Protein [alpha] Subunits in Escherichia coli / Maurine E. Linder -- Ch. 2. Purification of Recombinant G Protein [alpha] and [beta][gamma] Subunits from Sf9 Cells / Tohru Kozasa -- Ch. 3. Expression and Functional Analysis of G Protein [alpha] Subunits in Mammalian Cells / Catherine H. Berlot -- Ch. 4. Identification of Regulators and Targets of G Protein [alpha] Subunits Using the Yeast Two-Hybrid System / Jennifer L. Glick, Thomas E. Meigs and Patrick J. Casey -- Ch. 5. Functional Analysis of G Protein [beta][gamma] Subunits / K. Yan, O. G. Kisselev and A. N. Pronin / [et al.] -- Ch. 6. Immunological Techniques of G Protein Analysis / Catherine A. Chen and David R. Manning -- Ch. 7. GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins / Jun Wang, Yaping Tu and Suchetana Mukhopadhyay / [et al.] -- Ch. 8. Fatty Acid Acylation of G Protein [alpha] Subunits / Philip B. Wedegaertner --
  2. Ch. 9. ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin / Marilyn J. Woolkalis -- Ch. 10. Analysis of Receptor-G Protein Coupling with Pertussis Toxin-Resistant Mutants of G[subscript i] and G[subscript o] / Albrecht Moritz and Ronald Taussig -- Ch. 11. Microinjection of Antibodies Neutralizing G Protein Function / Vickie J. LaMorte, James R. Feramisco and Judy L. Meinkoth -- Ch. 12. Agonist-Promoted [[superscript 35]S]GTP[gamma]S-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells / Alastair J. Barr and David R. Manning -- Ch. 13. Methods of Sequestering [beta][gamma] in the Intact Cell / Gregory J. Della Rocca, Robert J. Lefkowitz and Walter J. Koch -- Ch. 14. In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies / Hsien-yu Wang, Xunxian Liu and Zhiyong Cheng / [et al.].
Owning institution
  1. Columbia University Libraries
Bibliography (note)
  1. Includes bibliographical references and index.