Research Catalog

Details

Additional authors

1. Reed, John C., 1958-

Description

1. xxxiii, 569 pages : illustrations; 24 cm.

Series statement

1. Methods in enzymology ; v. 322

Uniform title

1. Methods in enzymology ; v. 322.

Subject

1. Cellules > Mort
2. Apoptose
3. Cell death
4. Apoptosis

Contents

1. Sect. I. Measuring Apoptosis and Apoptosis-Induced Endonucleases. 1. Detection of DNA Cleavage in Apoptotic Cells / Scott H. Kaufmann, Peter W. Mesner, Jr. and Kumiko Samejima / [et al.]. 2. Detection of Apoptosis by Annexin V Labeling / Ella Bossy-Wetzel and Douglas R. Green. 3. Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry / Zbigniew Darzynkiewicz and Elzbieta Bedner. 4. Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes / Michelle Barry, Jeffrey Heibein and Michael Pinkoski / [et al.]. 5. Apoptotic Nuclease Assays / Francis M. Hughes, Jr. and John A. Cidlowski -- Sect. II. Measuring Apoptosis in Lower Organisms. 6. Analysis of Programmed Cell Death and Apoptosis in Drosophila / Po Chen and John M. Abrams. 7. Programmed Cell Death in the Nematode Caenorhabditis elegans / Duncan Ledwich, Yi-Chun Wu and Monica Driscoll / [et al.] -- Sect. III. Proteases Involved in Apoptosis and Their Inhibitors.
2. 8. Caspase Assays / Henning R. Stennicke and Guy S. Salvesen. 9. Determination of Caspase Specificities Using a Peptide Combinatorial Library / Nancy A. Thornberry, Kevin T. Chapman and Donald W. Nicholson. 10. Criteria for Identifying Authentic Caspase Substrates during Apoptosis / Sophie Roy and Donald W. Nicholson. 11. Purification and Use of Granzyme B / Lianfa Siu, Xiaohe Yang and Christopher J. Froelich / [et al.]. 12. Viral Caspase Inhibitors CrmA and p35 / Qiao Zhou and Guy S. Salvesen. 13. Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase Inhibitors / Quinn L. Deveraux, Kate Welsh and John C. Reed. 14. Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae / Christine J. Hawkins, Susan L. Wang and Bruce A. Hay -- Sect. IV. Cell-Free Systems for Monitoring Steps in Apoptosis Pathways. 15. In Vitro Assays for Caspase-3 Activation and DNA Fragmentation / Xuesong Liu and Xiaodong Wang.
3. 16. Cell-Free Apoptosis in Xenopus laevis Egg Extracts / Oliver von Ahsen and Donald D. Newmeyer. 17. Cytofluorometric Quantification of Nuclear Apoptosis Induced in a Cell-Free System / Hans K. Lorenzo, Santos A. Susin and Guido Kroemer -- Sect. V. Mitochondria and Apoptosis. 18. Purification of Mitochondria for Apoptosis Assays / Santos A. Susin, Nathanael Larochette and Maurice Geuskens / [et al.]. 19. Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated Mitochondria / Naoufal Zamzami, Didier Metivier and Guido Kroemer. 20. Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells / Roberta A. Gottlieb and Souichi Adachi. 21. Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabilized Cells / Gary Fiskum, Alicia J. Kowaltowski and Alexander Y. Andreyev / [et al.]. 22. Assays for Cytochrome c Release from Mitochondria during Apoptosis / Ella Bossy-Wetzel and Douglas R. Green.
4. 23. Purification and Liposomal Reconstitution of Permeability Transition Pore Complex / Catherine Brenner, Isabel Marzo and Helena L. De / [et al.] -- Sect. VI. Bcl-2 Family Proteins. 24. Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate Assays / Jose-Luis Diaz, Tilman Oltersdorf and Lawrence C. Fritz. 25. Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance / Zhihua Xie and John C. Reed. 26. Measuring Pore Formation by Bcl-2 Family Proteins / Sharon L. Schendel and John C. Reed. 27. Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cerevisiae / Qunli Xu, Ning Ke and Shigemi Matsuyama / [et al.]. 28. Exploiting the Utility of Yeast in the Context of Programmed Cell Death / Catherine N. Torgler, Robin Brown and Eric Meldrum -- Sect. VII. Studying Receptors and Signal Transduction Events Implicated in Cell Survival and Cell Death.
5. 29. Production of Recombinant TRAIL and TRAIL Receptor:Fc Chimeric Proteins / Pascal Schneider. 30. Expression of Lymphotoxins and Their Receptor-Fc Fusion Proteins by Baculovirus / Isabelle Rooney, Kristine Butrovich and Carl F. Ware. 31. Analysis of the CD95 (APO-1/Fas) Death-Inducing Signaling Complex by High-Resolution Two-Dimensional Gel Electrophoresis / Carsten Scaffidi, Frank C. Kischkel and Peter H. Krammer / [et al.]. 32. Measurement of Ceramide Levels by the Diacylglyccrol Kinase Reaction and by High-Performance Liquid Chromatography-Fluorescence Spectrometry.

Owning institution

1. Columbia University Libraries

Bibliography (note)

1. Includes bibliographical references and indexes.