DNA sequencing : from experimental methods to bioinformatics
- Title
- DNA sequencing : from experimental methods to bioinformatics / Luke Alphey.
- Published by
- New York : Springer, 1997.
- Author
Items in the library and off-site
Displaying 1 item
| Status | Format | Access | Call number | Item location |
|---|---|---|---|---|
| Status | FormatText | AccessUse in library | Call numberQP625.N89 A45 1997 | Item locationOff-site |
Details
- Description
- xiii, 206 p. : ill.; 24 cm.
- Summary
- This practical guide presents all aspects of DNA sequencing - from descriptions of the basic methods, to key applications, to the analysis of sequence data. By explaining the options available and their relative merits, DNA Sequencing allows newcomers to the field to decide which method is most suitable for their application. For experienced sequencers, the book is a valuable reference source for details of less common techniques and as a means of updating their knowledge.
- Series statement
- Introduction to biotechniques
- Uniform title
- Introduction to biotechniques series.
- Subject
- Contents
- 1. What is DNA Sequencing? -- 2. Chemical Degradation (Maxam and Gilbert) Method -- 3. Chain Termination (Sanger Dideoxy) Method -- 4. Instrumentation and Reagents -- 5. Template Preparation -- 6. Gel Electrophoresis -- 7. Nonradioactive Methods -- 8. Troubleshooting -- 9. Confirmatory Sequencing -- 10. Sequencing PCR Products -- 11. Strategies for New Sequence Determination -- 12. Introduction to Bioninformatics and the Internet / A. Brass -- 13. Sequence Databases / A. Brass -- 14. Sequence Alignment and Database Searches / A. Brass -- 15. Sequencing Projects and Contig Analysis / A. Brass -- 16. Protein Function Prediction / A. Brass -- 17. Protein Structure Prediction / A. Brass -- App. B. Amino acid and nucleotide codes -- App. C. Suppliers.
- Owning institution
- Columbia University Libraries
- Bibliography (note)
- Includes bibliographical references and index.