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E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Preston.

Title
  1. E. coli plasmid vectors : methods and applications / edited by Nicola Casali and Andrew Preston.
Published by
  1. Totowa, N.J. : Humana Press, 2003.

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Details

Additional authors
  1. Casali, Nicola.
  2. Preston, Andrew, 1969-
Description
  1. xi, 316 p. : ill.; 24 cm.
Series statement
  1. Methods in molecular biology ; v. 235
Uniform title
  1. Methods in molecular biology (Clifton, N.J.) ; v. 235.
Subject
  1. Escherichia coli
  2. Laboratory Manual
  3. Escherichia coli > Laboratory manuals
  4. Genetic vectors > Laboratory manuals
  5. Genetic Vectors
  6. Plasmids > Laboratory manuals
Genre/Form
  1. Laboratory Manual.
Contents
  1. 1. The Function and Organization of Plasmids / Finbarr Hayes -- 2. Choosing a Cloning Vector / Andrew Preston -- 3. Escherichia coli Host Strains / Nicola Casali -- 4. Chemical Transformation of E. coli / W. Edward Swords -- 5. Electroporation of E. coli / Claire A. Woodall -- 6. DNA Transfer by Bacterial Conjugation / Claire A. Woodall -- 7. Cosmid Packaging and Infection of E. coli / Mallory J. A. White and Wade A. Nichols -- 8. Isolation of Plasmids from E. coli by Alkaline Lysis / Sabine Ehrt and Dirk Schnappinger -- 9. Isolation of Plasmids from E. coli by Boiling Lysis / Sabine Ehrt and Dirk Schnappinger -- 10. High-Purity Plasmid Isolation Using Silica Oxide / Stefan Grimm and Frank Voss-Neudecker -- 11. High-Throughput Plasmid Extraction Using Microtiter Plates / Michael A. Quail -- 12. Isolation of Cosmid and BAC DNA from E. coli / Daniel Sinnett and Alexandre Montpetit -- 13. Preparation of Single-Stranded DNA from Phagemid Vectors / W. Edward Swords -- 14. Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects / Robert H. Gross -- 15. Cloning in Plasmid Vectors / Carey Pashley and Sharon Kendall -- 16. Extraction of DNA from Agarose Gels / Nicholas Downey -- 17. Cloning PCR Products with T-Vectors / Wade A. Nichols -- 18. Construction of Genomic Libraries in [Lambda]-Vectors / Yilun Wang, Zheng Cao, Darryl Hood and James G. Townsel -- 19. Rapid Screening of Recombinant Plasmids / Sangwei Lu -- 20. Restriction Analysis of Recombinant Plasmids / Joanne Goranson-Siekierke and Jarrod L. Erbe -- 21. Screening Recombinant DNA Libraries / Wade A. Nichols -- 22. Sequencing Using Fluorescent-Labeled Nucleotides / Allison F. Gillaspy -- 23. Site-Directed Mutagenesis Using the Megaprimer Method / Zhidong Xu, Alessia Colosimo and Dieter C. Gruenert -- 24. Site-Directed Mutagenesis by Inverse PCR / Clifford N. Dominy and David W. Andrews -- 25. Creating Nested DNA Deletions Using Exonuclease III / Rosamund Powles and Lafras M. Steyn -- 26. Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs / Alistair McGregor -- 27. In Vitro Transcription and Translation / Farahnaz Movahedzadeh, Susana Gonzalez Rico and Robert A. Cox -- 28. Vectors for the Expression of Recombinant Proteins in E. coli / Sally A. Cantrell -- 29. Expression of Recombinant Proteins from lac Promoters / Charles R. Sweet -- 30. Plasmid-Based Reporter Genes: Assays for [beta]-Galactosidase and Alkaline Phosphatase Activities / Minghsun Liu -- 31. Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein / Sergei R. Doulatov.
Owning institution
  1. Columbia University Libraries
Bibliography (note)
  1. Includes bibliographical references and index.