G protein signaling : methods and protocols / edited by Alan V. Smrcka.
- Title
- G protein signaling : methods and protocols / edited by Alan V. Smrcka.
- Published by
- Totowa, NJ : Humana Press, 2004.
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Displaying 1 item
Status | Format | Access | Call number | Item location |
---|---|---|---|---|
Status | FormatText | AccessRequest in advance | Call numberQH506 .M56 v.237 | Item locationOff-site |
Details
- Additional authors
- Description
- xii, 249 p. : ill.; 24 cm.
- Series statement
- Methods in molecular biology ; v. 237
- Uniform title
- Methods in molecular biology ; v. 237.
- Subject
- Genre/Form
- Laboratory Manual.
- Contents
- 1. Purification of Recombinant G Protein [alpha] Subunits from Escherichia coli / Wendy K. Greentree and Maurine E. Linder -- 2. Purification of G protein Subunits form Sf9 Insect Cells Using Hexahistidine-Tagged [alpha] and [beta][gamma] Subunits / Tohru Kozasa -- 3. Expression and Purification of Soluble Adenylyl Cyclase from Eschericia coli / Jeff A. Beeler and Wei-Jen Tang -- 4. Purification of Phospholipase C [beta] and Phospholipase C [epsilon] from Sf9 Cells / Mousumi Ghosh, Huan Wang, Grant G. Kelley and Alan V. Smrcka -- 5. Assay for G Protein-Dependent Activation of Phospholipase C [beta] Using Purified Protein Components / Mousumi Ghosh and Alan V. Smrcka -- 6. Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells / Ronald Taussig -- 7. Measurement of G Protein-Coupled Receptor-Stimulated Phospholipase D Activity in Intact Cells / Stephanie J. Walker and H. Alex Brown -- 8. Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells / Bo Liu and Dianqing Wu -- 9. Intensive Mutational Analysis of G Protein-Coupled Receptors in Yeast / Andjelka Celic, Sara M. Connelly, Negin P. Martin and Mark E. Dumont -- 10. Green Fluorescent Protein-Tagged [beta]-Arrestin Translocation as a Measure of G Protein-Coupled Receptor Activation / Stephen S. G. Ferguson and Marc G. Caron -- 11. Detection of G Protein-Coupled Receptors by Immunofluorescence Microscopy / Patricia M. Hinkle and John A. Puskas -- 12. Assay of G Protein-Coupled Receptor Activation of G Proteins in Native Cell Membranes Using [[superscript S]]GTP[gamma]S Binding / Jean M. Bidlack and Amy L. Parkhill -- 13. Analysis of the Coupling of G[subscript 12/13] to G Protein-Coupled Receptors Using a Luciferase Reporter Assay / Bo Liu and Dianqing Wu -- 14. Selective Inhibition of G Protein-Mediated Pathways Using RGS Domains / Tohru Kozasa and Richard D. Ye -- 15. Ribozymes as Tools for Suppression of G Protein [gamma] Subunits / Janet D. Robishaw, Zheng-ping Guo and Qin Wang -- 16. In Vivo Adenoviral-Mediated Gene Transfer of the [beta]ARKct to Study the Role of G[beta][gamma] in Arterial Restenosis / Guido Iaccarino and Walter J. Koch -- 17. Analysis of RGS Protein Palmitoylation / Leah S. Bernstein, Maurine E. Linder and John R. Hepler -- 18. Methods for Measuring RGS Protein Phosphorylation by G Protein-Regulated Kinases / Susanne Hollinger and John R. Hepler -- 19. The Use of Green Fluorescent Proteins to View Association Between Phospholipase C[beta] and G Protein Subunits in Cells / Suzanne Scarlata and Louisa Dowal -- 20. Cellular Localization of GFP-Tagged [alpha] Subunits / Thomas R. Hynes, Thomas E. Hughes and Catherine H. Berlot.
- Owning institution
- Columbia University Libraries
- Bibliography (note)
- Includes bibliographical references and index.