Research Catalog

Details

Additional authors

1. Dickson, Robert C.
2. Mendenhall, Michael Dean.

Description

1. xii, 327 p. : ill.; 24 cm.

Series statement

1. Methods in molecular biology ; 284

Uniform title

1. Methods in molecular biology (Clifton, N.J.) ; v. 284.

Subject

1. Cellular signal transduction > Laboratory manuals
2. GTP-Binding Proteins > analysis
3. Cell receptors > Laboratory manuals
4. Laboratory Manual
5. G proteins > Laboratory manuals
6. Signal Transduction > physiology

Genre/Form

1. Laboratory Manual.

Contents

1. 1. Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- 2. Signal transduction inhibitors in cellular function / Maofu Fu, Chenguang Wang, Xueping Zhang and Richard G. Pestell -- 3. Two-dimensional gel electrophoresis for the identification of signaling targets / Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing and Natalie G. Ahn -- 4. A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry and Michelle L. Twaroski -- 5. Determing protein half-lives / Pengbo Zhou -- 6. Assaying protein kinase activity / Jan Brabek and Steven K. Hanks -- 7. Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- 8. Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- 9. Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation / Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu and Emery H. Bresnick -- 10. Characterization of protein-DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- 11. Nonradioactive methods for detecting activation of Ras-related small G proteins / Douglas A. Andres -- 12. Nucleocytoplasmic glycosylation, O-GIcNAc : identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung and Gerald Warren Hart -- 13. Techniques in protein methylation / Jaeho Lee, Donghang Cheng and Mark T. Bedford -- 14. Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- 15. Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- 16. Assaying phospholipase A[subscript 2] activity / Christina C. Leslie and Michael H. Gelb -- 17. Measurement and immunofluorescence of cellular phosphoinositides / Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich and Daryll B. DeWald -- 18. Measuring dynamic changes in cAMP using fluorescence resonance energy transfer / Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron and Manuela Zaccolo -- 19. In vivo detection of protein-protein interaction in plant cells using BRET / Chitra Subramanian, Yao Xu, Carl Hirschie Johnson and Albrecht G. von Arnim -- 20. Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- 21. Assaying cytochrome c translocation during apoptosis / Nigel J. Waterhouse, Rohan Steel, Ruth Kluck and Joseph A. Trapani.

Owning institution

1. Columbia University Libraries

Bibliography (note)

1. Includes bibliographical references and index.