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DNA sequencing : optimizing the process and analysis

Title

DNA sequencing : optimizing the process and analysis / edited by Jan Kieleczawa.

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Status	Format	Access	Call number	Item location
Status Request for on-site use Request scan How do I pick up this item and when will it be ready?	FormatText	AccessRequest in advance	Call numberQP624 .D1748 2005	Item locationOff-site
Status Request for on-site use Request scan How do I pick up this item and when will it be ready?	FormatText	AccessRequest in advance	Call numberQP624 .D1748 2005	Item locationOff-site

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Summary

"DNA Sequencing: Optimizing the Process and Analysis was written to help scientists sequence difficult templates. It is also a practical guide to faster and more efficient routine DNA sequencing. This in-depth volume covers important topics in the field, including biochemical and technological advances stemming from the Human Genome Project, proven and newly emerging methods of preparing DNA templates, the effects of widely used laboratory reagents on DNA sequencing, Laboratory Information Management Systems (LIMS), setting up of a GLP/GMP DNA sequencing lab, and the future of DNA sequencing."--BOOK JACKET.

Subject

Contents

Foreword / Elaine R. Mardis -- Ch. 1. Controlled heat-denaturation of DNA plasmids / Jan Kieleczawa -- Ch. 2. Effects of various reagents on DNA sequencing process / Jan Kieleczawa -- Ch. 3. Sequencing of difficult DNA templates / Jan Kieleczawa -- Ch. 4. New DNA sequencing enzymes / Sandra L. Spurgeon and John W. Brandis -- Ch. 5. Beyond pUC : vectors for cloning unstable DNA / Ronald Godiska, Melodee Patterson, Tom Schoenfeld and David A. Mead -- Ch. 6. Recombination-based cloning / Vincent Ling -- Ch. 7. Plasmid preparation methods for DNA sequencing / Parke K. Flick -- Ch. 8. Optimization of culture growth in 96-deep-well plates / Jan Kieleczawa -- Ch. 9. Automated DNA scanners used in sequencing laboratories / Jan Kieleczawa -- Ch. 10. Geospiza's finch-server : a complete data management system for DNA sequencing / Sandra Porter, Joe Slagel and Todd Smith -- Ch. 11. DNA sequencing database : a flexible LIMS for DNA sequencing analysis / Donald M. Koffman and Hemchand Sookdeo -- Ch. 12. Good laboratory practices, good manufacturing procedures, and quality assurance in the DNA sequencing laboratory / Michele Godlevski and Thalia Taylor -- Ch. 13. Future of DNA sequencing : towards an affordable genome / Kevin McKernan.

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