Research Catalog

Calcium signaling protocols

Title
  1. Calcium signaling protocols / edited by David G. Lambert.
Published by
  1. Totowa, N.J. : Humana Press, [2006], ©2006.

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Details

Additional authors
  1. Lambert, David G.
Description
  1. x, 359 pages : illustrations; 24 cm.
Series statement
  1. Methods in molecular biology ; v. 312
Uniform title
  1. Methods in molecular biology (Clifton, N.J.) ; v. 312.
Subject
  1. Spectrometry, Fluorescence > methods
  2. Calcium > Research > Methodology
  3. Calcium Channels > pharmacokinetics
  4. Calcium > Laboratory manuals
  5. Calcium channels > Research > Methodology
  6. Calcium Signaling > physiology
  7. Signal Transduction
  8. Calcium > pharmacokinetics
  9. Cellular signal transduction > Research > Methodology
Contents
  1. Fluorescent measurement of [Ca2+]c: basic practical considerations / Alec W.M. Simpson -- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst ... [et al.] -- Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans -- Confocal microscopy: theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars -- Single cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas -- Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer
  2. Measurement of Ca2+ entry into cells using 45Ca2+ / Mercedes Villarroya ... [et al.] -- Measurement of [3H]PN200-110 and [125I]--conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert -- Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents in single cells: heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani -- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft -- Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart --
  3. Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox -- Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann -- Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer -- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide -- Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn --
  4. Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan ... [et al.] -- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng -- Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma -- Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma -- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka.
Owning institution
  1. Columbia University Libraries
Bibliography (note)
  1. Includes bibliographical references and index.