Research Catalog

Details

Additional authors

1. Starkey, Michael P.
2. Elaswarapu, Ramnath.

Description

1. xv, 425 pages : illustrations; 25 cm.

Series statement

1. Methods in molecular biology ; 439

Uniform title

1. Methods in molecular biology (Clifton, N.J.) ; v. 439.

Subject

1. Genetic Techniques
2. Molecular genetics > Laboratory manuals
3. Laboratory Manual
4. Genomics > methods
5. Sequence Analysis, DNA > methods
6. Molecular Biology > methods
7. Chromosome Mapping > methods

Genre/Form

1. Laboratory Manual.

Contents

1. Chapter 1. Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield / Kaisa Silander and Janna Saarela -- Chapter 2. Scanning for DNA Variants by Denaturant Capillary Electrophoresis / Per O. Ekstrom -- Chapter 3. Identification of SNPs, or Mutations in Sequence Chromatograms / Nicole Draper -- Chapter 4. BeadArray-Based Genotyping / Helen Butler and Jiannis Ragoussis -- Chapter 5. Microsatellite-Based Candidate Gene Linkage Analysis Studies / Cathryn Mellersh -- Chapter 6. Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) / Alexei Protopopov, Bin Feng and Lynda Chin -- Chapter 7. Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification / Minoru Takata -- Chapter 8. Application of Microarrays for DNA Methylation Profiling / Axel Schumacher, Andreas Weinhausl and Arturas Petronis -- Chapter 9. Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray / Byung-Kwan Cho, Eric M. Knight and Bernhard O. Palsson -- Chapter 10. Transcriptional Profiling of Small Samples in the Central Nervous System / Stephen D. Ginsberg -- Chapter 11. Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays / Marina Bibikova, Joanne M. Yeakley, Jessica Wang-Rodriguez and Jian-Bing Fan -- Chapter 12. Expression Profiling of microRNAs in Cancer Cells: Technical Considerations / Mouldy Sioud and Lina Cekaite -- Chapter 13. Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry / Sigrun Langbein -- Chapter 14. The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease / Peter Ellmark, Adrian Woolfson, Larissa Belov and Richard I. Christopherson -- Chapter 15. Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis / Gert Van den Bergh and Lutgarde Arckens -- Chapter 16. Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags / Arsalan S. Haqqani, John F. Kelly and Danica B. Stanimirovic -- Chapter 17. Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics / Arsalan S. Haqqani, John F. Kelly and Danica B. Stanimirovic -- Chapter 18. Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line / John F. Kelly and Wen Ding -- Chapter 19. Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression / Binesh Shrestha, Carol Smee and Opher Gileadi -- Chapter 20. Coimmunoprecipitation and Proteomic Analyses / S. Fabio Falsone, Bernd Gesslbauer and Andreas J. Kungl -- Chapter 21. Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes / Peter Kaiser, David Meierhofer, Xiaorong Wang and Lan Huang -- Chapter 22. Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in vivo / Runtao He and Xuguang Li -- Chapter 23. Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation / Victor Villalobos, Snehal Naik and David Piwnica-Worms -- Chapter 24. Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells / John E. Collins -- Chapter 25. GeneFAS: A Tool for the Prediction of Gene Function Using Multiple Sources of Data / Trupti Joshi, Chao Zhang, Guan Ning Lin, Zhao Song and Dong Xu -- Chapter 26. Comparative Genomics-Based Prediction of Protein Function / Toni Gabaldon -- Chapter 27. Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing / Louise A. Dawson and Badar A. Usmani.

Owning institution

1. Columbia University Libraries

Bibliography (note)

1. Includes bibliographical references and index.