Research Catalog

Details

Additional authors

1. Dunbar, Bonnie S.

Description

1. xxiii, 242 p. : ill.; 24 cm.

Summary

1. Protein blotting is a technique of major importance within the life sciences. While the most commonly used method is the use of antibodies to detect protein antigens, in recent years this technology has been expanded to characterize a wide range of different interactions between proteins and other proteins, carbohydrates, and DNA. This book, written by experts in the field, presents in detail all the key techniques used for protein blotting. It describes the basic equipment required, principles, methods for preparing samples for protein transfer, and detection methods such as radiometric and bioluminescence-enhanced detection systems. Important applications are also discussed, including the detection and characterization of glycoprotein carbohydrate chains, and the study of DNA binding of immobilized proteins. Protein Blotting: A Practical Approach is a comprehensive practical guide for all those using this technique in the fields of molecular biology and biotechnology, and in biomedical and pharmaceutical research in general.

Series statement

1. Practical approach series

Uniform title

1. Practical approach series

Subject

1. Western immunoblotting > Laboratory manuals
2. Blotting, Western
3. Proteins > analysis

Genre/Form

1. Handbooks and manuals
2. Laboratory manuals

Contents

1. Historical introduction / J.M. Gershoni -- Basic equipment and methods for protein blotting -- Introduction and basic set-up for protein transfer / Bonnie S. Dunbar -- Protein blotting and immunoblotting using nitrocellulose membranes / Antonio De Maio -- Protein blotting using polyvinylidene fluoride membranes / Michael A. Mansfield -- Protein blotting using semi-dry electrophoretic transfer equipment / Gunilla Jacobson.
2. (cont) Principles and methods for preparing samples for protein transfer -- Transfer and blocking conditions in immunoblotting / A. Van Dam -- Sample preparation for protein electrophoresis and transfer / Vaughan H. Lee, Bonnie S. Dunbar -- Strategy for purification of organelles from parasites: protein blotting as one of the initial steps / David W. Sammons -- Detection methods and uses in immunoblotting -- Radiometric methods for detection in blots / R.A. Laskey.
3. (cont) Bioluminescence-enhanced detection systems in protein blotting / Reinhard Erich Geiger -- Chemiluminescent detection systems for protein blotting / Ian Durrant, Sue Fowler -- Applications of protein blotting -- Detection and characterization of glycoprotein carbohydrate chains after electrophoretic separation / Tatsuro Irimura, Hiroto Kawashima -- DNA binding of immobilized proteins / Constantin N. Flytzanis.
4. (cont) Use of peptide ligands for the detection of binding proteins by western blotting / Paul Hossenlopp, Michel Binoux -- Use of blotted proteins as immunogens / Boguslaw Szewczyk, David R. Harper -- Amino acid sequence analysis of blotted proteins / Richard G. Cook -- Renaturative catalytic blotting of enzyme proteins / Jia-Shi Zhu, Gary M. Gray.

Owning institution

1. Harvard Library

Bibliography (note)

1. Includes bibliographical references and index.

Processing action (note)

1. committed to retain