Research Catalog

Details

Additional authors

1. Potrykus, I. (Ingo), 1933-
2. Spangenberg, G. (German), 1959-

Description

1. xxii, 361 pages : illustrations; 25 cm

Summary

1. The clearly structured protocols given in this manual allow the application of the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics. Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.

Series statement

1. Springer lab manual

Uniform title

1. Springer lab manual

Subject

1. Plant genetic engineering > Laboratory manuals
2. Crops > Genetic engineering > Laboratory manuals
3. Plant genetic transformation > Laboratory manuals
4. Crops > Genetic engineering
5. Plant genetic engineering
6. Plant genetic transformation
7. Gentechnologie
8. Gentransfer
9. Pflanzen
10. Transgene Pflanzen
11. Genetische manipulatie
12. Planten
13. Plantes > Transformation génétique
14. Transformation génétique > Manuels de laboratoire
15. Plantes > Génie génétique
16. Cultures > Génie génétique > Manuels de laboratoire

Genre/Form

1. Laboratory Manual
2. Laboratory manuals.
3. Manuels de laboratoire.
4. Aufsatzsammlung.

Contents

1. Pt. I. Agrobacterium tumefaciens-Mediated Gene Transfer -- Introduction I Agrobacterium tumefaciens, a Natural Vector System / P. J. J. Hooykaas. 1. Transformation of Arabidopsis thaliana Using Root Explants / D. Valvekens, A. Van Gysel, M. Van Montagu and M. Van Lijsebettens. 2. Seed Transformation in Arabidopsis thaliana / K. A. Feldmann. 3. In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum-Infiltration / N. Bechtold and D. Bouchez. 4. Agrobacterium-Mediated Transformation of Potato (Solanum tuberosum) / J. Dietze, A. Blau and L. Willmitzer. 5. Agrobacterium-Mediated Transformation of Rapeseed (Brassica napus) / J. B. Bade and B. Damm -- Pt. II. Agrobacterium rhizogenes-Mediated Gene Transfer -- Introduction II Agrobacterium rhizogenes, a Natural Transformation System / D. Tepfer. 6. Agrobacterium rhizogenes-Mediated Transformation: Transformed Roots to Transformed Plants / D. Tepfer -- Pt. III. Direct Gene Transfer to Protoplasts -- Introduction III Direct Gene Transfer to Protoplasts / I. Potrykus. 7. Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants / G. Spangenberg and I. Potrykus. 8. Polyethylene Glycol-Mediated Direct Gene Transfer to Indica Rice Protoplasts and Regeneration of Transgenic Plants / S. K. Datta. 9. Plastid Transformation by Polyethylene Glycol Treatment of Protoplasts and Regeneration of Transplastomic Tobacco Plants / H. U. Koop and W. Kofer. 10. Electroporation-Mediated Gene Transfer to Protoplasts and Regeneration of Transgenic Plants / M. G. K. Jones. 11. Electroporation Mediated Gene Transfer to Japonica Rice Protoplasts and Regeneration of Transgenic Plants / C. Wu and K. Shimamoto. 12. Regeneration of Transgenic Maize Plants from Embryogenic Protoplasts After Polyethylene Glycol-Mediated DNA Uptake / S. Omirulleh, S. Morocz and D. Dudits. 13. Gene Transfer to Protoplasts: Transient Gene Expression Analysis / J. C. Carle Urioste, K. Marrs, J. Bodeau and V. Walbot -- Pt. IV. Biolistic Plant Transformation -- Introduction IV The Biolistic Transformation System / T. Klein. 14. Transient Gene Expression Studies with the Biolistic System / D. A. Russell and M. E. Fromm. 15. Maize Transformation by Microprojectile Bombardment of Immature Embryos / E. Dunder, J. Dawson, J. Suttie and G. Pace. 16. Biolistic Transformation of Microspore-Derived and Immature Zygotic Embryos and Regeneration of Fertile Transgenic Barley Plants / Y. Wan and P. G. Lemaux. 17. Transformation of Soybean (Glycine max) Through Electric Discharge Particle Acceleration / P. Christou. 18. Ballistic Microtargeting of Visible Marker Genes to the Shoot Meristem of Wheat / C. Sautter and R. Bilang. 19. Stable Transformation of Wheat by Microprojectile Bombardment / J. T. Weeks. 20. Biolistic-Based Analysis of Chloroplast Gene Expression / U. Klein and L. Bogorad -- Pt. V. Microinjection and Fiber-Mediated Transformation -- Introduction V Microinjection into Plant Cells: Methodology and Applications / G. Neuhaus. 21. Microinjection into Tobacco Protoplasts and Regeneration of Transgenic Plants / M. Schnorf, B. Kost, A. Galli and G. Neuhaus. 22. Silicon Carbide Whisker-Mediated Transformation: Regeneration of Transgenic Maize Plants / K. Wang, B. R. Frame, P. R. Drayton and J. A. Thompson -- Pt. VI. Tissue Electroporation -- Introduction VI Transformation by Tissue-Electroporation / I. Potrykus. 23. Transient Expression Following Electroporation into Immature Zygotic Embryos of Wheat / A. Kloti. 24. Transgenic Indica Rice Plants by Electroporation of Seed Embryo Cells / X. Xu, H. Shi and B. Li -- Pt. VII. Analysis of Transgenic Plants -- Introduction VII Analysis of Transgenic Plants / I. Negrutiu. 25. Standard Molecular Techniques for the Analysis of Transgenic Plants / J. Futterer, A. Gisel, V. Iglesias, A. Kloti, B. Kost, O. Mittelsten Scheid, G. Neuhaus, G. Neuhaus-Url, M. Schrott, R. Shillito, G. Spangenberg and Z. Y. Wang. 26. In Situ Hybridization on Plant Metaphase Chromosomes / I. Farbos and A. Mouras. 27. Localisation of RNA Transcripts in Plant Tissue by in Situ Hybridisation / A. Y. Fleming. 28. In Situ Immunolocalisation of Transgene Products in Transgenic Plants / S. Craig and T. J. V. Higgins -- Pt. VIII. Establishment and Maintenance of Embryogenic Cultures -- Introduction VIII Embryogenic Cultures: a Source of Target Cells for Gene Transfer / G. Spangenberg. 29. Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and Their Protoplasts in Forage Grasses / Z. Y. Wang, G. Legris and G. Spangenberg -- Pt. IX. Genetic Markers and Expression Signals -- Introduction IX Genetic Markers and Expression Signals / J. Futterer. 30. Expression Signals and Vectors / J. Futterer. 31. Selectable Marker and Reporter Genes / M. Schrott -- Pt. X. Guidelines and Biosafety Considerations -- Introduction X Gene Technology with Plants and the Public / I. Potrykus. 32. Release of Transgenic Plants: Regulations and Developments / J. Landsmann. 33. Risk Assessment / K. Schluter.

Owning institution

1. Princeton University Library

Bibliography (note)

1. Includes bibliographical references.