Research Catalog

Details

Additional authors

1. Li, Guangpu (Molecular biologist)
2. Segev, Nava

Description

1. xii, 310 pages : illustrations (some color); 26 cm.

Summary

1. This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.

Series statement

1. Methods in molecular biology, 1064-3745 ; 2293
2. Springer protocols

Uniform title

1. Methods in molecular biology (Clifton, N.J.) ; v. 2293.
2. Springer protocols (Series)

Subject

1. G proteins
2. Guanosine triphosphatase
3. GTP-Binding Proteins
4. GTP Phosphohydrolases

Contents

1. Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview -- Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase -- High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins -- Detecting Endogenous Rab8 Activation -- Testing the Phenotypic Effects of a Rab Chimera that Resolves Exchange Factor Specificity from Effector Specificity -- Profiling Structural Alterations during Rab5 Nucleotide Exchange by HDX-MS -- CLEM Characterization of Rab8 and Associated Membrane Trafficking Regulators at Primary Cilium Structures -- Imaging of Spatial Cycling of Rab GTPase in the Cell -- Deconvolution of Multiple Rab Binding Domains using the Batch Yeast 2-Hybrid Method DEEPN -- Determination of the Rab27-Effector Binding Affinity Using a High-Throughput FRET-Based Assay -- Methods to Study the Unique SOCS Box Domain of the Rab40 Small GTPase Subfamily -- Using GBP Nanotrap to Restore Autophagy in the Rab5/Vps21 Mutant by Forcing Snf7 and Atg17 Interaction -- Establishing Regulation of a Dynamic Process by Ypt/Rab GTPase: A Case for Cisternal Progression -- Methods for Assessing the Regulation of a Kinase by the Rab GTPase Ypt1 -- Qualitative and Quantitative Assessment of the Role of Endocytic Regulatory and/or Rab Proteins in Mitochondrial Fusion and Fission -- Characterization of the Role of Rab18 in Mediating LD-ER Contact and LD Growth -- Methods for Establishing Rab Knockout MDCK Cells -- Generating Rab6 Conditional Knockout Mice -- Use of Immunohistochemistry to Determine Expression of Rab5 Subfamily of GTPases in Mature and Developmental Brains -- Assessing Rab5 Activation in Health and Disease -- Quantitative Fluorescence Microscopy for Detecting Mammalian Rab Vesicles within the Parasitophorous Vacuole of the Human Pathogen Toxoplasma gondii.

Owning institution

1. Princeton University Library

Note

1. Formerly CIP.

Bibliography (note)

1. Includes bibliographical references and index.

Additional formats (note)

1. Also published electronically.

Terms of use (note)

1. Current copyright fee: GBP19.00